Metabolism is the most fundamental characteristic of life, with photosynthesis being the most important feature of plants, and respiratory metabolism being the most important feature of animals as heterotrophic organisms; The former (plants) absorbs CO₂And using solar energy to synthesize organic compounds (hydrocarbons such as starch) while releasing oxygen, the latter (animals) ingest organic compounds synthesized by plants while inhaling oxygen, then digest and decompose them, and exhale CO through respiration₂Usually, animals are CO treated for a certain period of time₂With O₂The ratio of consumption is called the respiratory quotient (RQ), which varies within a certain range depending on the composition of the animal's food. It can reflect which type of nutrient is the main source of energy for the animal at that time. If the main component of the food is sugar, the RQ is close to 1, and if the main component of the food is fat, the RQ is close to 0.7. By measuring animal CO₂The exhaled volume and oxygen consumption can be used to determine the respiratory metabolism of animals.

For insects, respiratory metabolism is also one of the important life characteristics. The energy obtained by insects from food (crops, fruits and vegetables, etc.) is released through respiration in the body, providing the necessary energy for life activities. The changes in insect respiratory metabolism can not only serve as representative physiological indicators, but also study the energy flow of the entire insect population, and have important application value in pest control. Many insect control methods aim to kill insects by causing metabolic abnormalities, so studying respiratory metabolism is of great significance for the study of insect physiology and ecology. The multi-channel insect respiratory metabolism measurement system can accurately measure the respiratory metabolism rate of multiple insects. Combined with certain experimental designs, it can study the effects of various factors (temperature, light, food type, developmental stage, oxygen concentration, insecticides, etc.) on respiratory metabolism, providing data support and guidance for pest control.

1、 Purpose:

The multi-channel insect respiratory metabolism measurement system consists of a carbon dioxide analyzer, an oxygen analyzer, a multi-channel gas path converter, an airflow controller, a data collector and program software, a breathing chamber, etc. A single channel to a maximum of 8 channels (capable of measuring respiratory metabolism of 7 animals simultaneously) system can be customized based on research content and budget, or CO can be measured simultaneously₂、 O₂、 RQ and H₂O， Alternatively, only CO measurement can be selected according to requirements₂Or O₂The measurement system. Used for accurately measuring the amount of carbon dioxide and oxygen consumption exhaled by various insects, and calculating respiratory quotient, it is widely used in research related to insect respiratory metabolism, such as insect physiology, insect ecology, genetics, pest control, and exercise physiology.

2、 Specific performance indicators:

1.Oxygen analyzer: Measurement technology: Fuel cell principle oxygen sensor; Range 0-100% (users can customize 5 levels); Accuracy of 0.1% (when O2 concentration is 2-100%); Resolution 0.0001% O2; Drift<0.01% per hour (under constant temperature conditions); Response time less than 7 seconds; 24-hour drift below 0.01%; 20 minutes of noise below 0.002% pk pk; Digital filtering (noise) adjustable from 0-50 seconds, amplification of 0.2 seconds, built-in A/D converter resolution of 16 bits; Temperature and pressure compensation; The temperature measurement range of the sensor is 0-60 ℃, with an accuracy of 0.2 ℃ and a resolution of 0.001 ℃; The resolution of atmospheric pressure measurement is 0.0001kPa, with an accuracy of 1% of the reading; Applicable flow range 5-2000mL/min; 4-channel analog signal output (0-5V BNC) can output oxygen concentration, atmospheric pressure, sensor temperature, user-defined; Digital output: RS-232； Equipped with a two-line text LCD display screen with backlight, it can simultaneously display oxygen content and air pressure; Power supply 12-15VDC, 25-150mA, equipped with AC adapter; Working temperature: 0-50 ℃, no condensation; Weight 2.8kg; Size 31cm × 10cm × 34cm

2.Dual channel high-precision differential oxygen analyzer (optional): Measurement technology: Fuel cell principle oxygen sensor, dual channel; Oxygen concentration range 0-100% (users can customize 5 levels); Difference range ± 50%; Accuracy of 0.1% (when O2 concentration is 2-100%); Resolution 0.0001% O2; Drift<0.01% per hour (under constant temperature conditions); Response time less than 7 seconds; 24-hour drift<0.01%; 20 minutes of noise<3ppm RMS; Digital filtering (noise) adjustable from 0-40 seconds, amplification of 0.2 seconds, built-in A/D converter resolution of 16 bits; Temperature and pressure compensation; The temperature measurement range of the sensor is 0-60 ℃, with an accuracy of 0.2 ℃ and a resolution of 0.001 ℃; The resolution of atmospheric pressure measurement is 0.0001kPa, with an accuracy of 0.05% of the full range; Applicable flow range 5-2000mL/min; 4-channel analog signal output (0-5V BNC) can output the oxygen concentration of channel 1, the oxygen concentration of channel 2, the difference between 1 and 2, and atmospheric pressure; Digital output: RS-232； Equipped with a 4-line text LCD display screen with backlight, it can simultaneously display the oxygen content and their difference, as well as atmospheric pressure of 2 channels; Unique PID (Proportional Integral Derivative) temperature control unit ensures constant temperature of the internal oxygen sensor, further improving the accuracy and stability of oxygen measurement; Power supply 12-24VDC, 8A, equipped with AC adapter; Working temperature: 5-45 ℃, no condensation; Weight 6.4kg; Size 43.2cm × 35.6cm × 20.3cm

3.Carbon dioxide analyzer: Measurement technology: Dual wavelength colorless dispersive infrared gas analyzer; Range 0-5% (Users can customize 9 levels. Other ranges can be customized); The accuracy is 1% of the reading; Resolution 0.0001% (1ppm); Drift<0.001% per hour (under constant temperature conditions); Response time less than 0.5 seconds; 24-hour drift below 0.002%; 20 minutes of noise<1 ppm RMS; Digital filtering (noise) adjustable from 0-50 seconds, amplification of 0.2 seconds, built-in A/D converter resolution of 16 bits; Temperature and pressure compensation; The resolution of atmospheric pressure measurement is 0.001kPa, with an accuracy of 1% of the reading; Applicable flow range 5-2000mL/min; 4-channel analog signal output (0-5V BNC) can output carbon dioxide concentration, atmospheric pressure, sensor temperature, user-defined; Digital output: RS-232； Equipped with a two-line text LCD display screen with backlight, it can simultaneously display oxygen content and air pressure; Power supply 12-15VDC, 175-250mA, equipped with AC adapter; Working temperature: 0-50 ℃, no condensation; Weight 2.8kg, size 31cm × 10cm × 34cm

4.Ultra high precision carbon dioxide analyzer (optional): used to measure the respiratory metabolism of small insects (such as flies, mosquitoes, etc.) or ticks and mites, and can simultaneously measure CO2 concentration and H2O concentration; CO2 range 0-3000ppm; Accuracy<1%; Resolution of 0.01ppm; H2O range 0-60mmol/mol; Accuracy of 1%;

5.Water vapor measuring instrument (optional), measurement technology: thin film capacitance principle; Range: 0-100% RH, accuracy 1%, resolution 0.001% RH; Dew point temperature range -40 to 40 ℃, accuracy 0.5 ℃, resolution 0.002 ℃; The range of water vapor density is 0-10ug/ml, with a resolution of 0.0001ug/ml; Water vapor pressure range 0-20000Pa, resolution 0.01Pa (when 0-1000Pa); Drift<0.01% RH per hour (under constant temperature conditions); Power supply 12-15VDC, 175-250mA, equipped with AC adapter; Working temperature: 0-50 ℃, no condensation; Applicable flow range 5-2000mL/min; 3-channel analog signal output (0-5V BNC) can output water vapor relative humidity% RH, dew point temperature (unit ℃ or ℉), and water vapor pressure (unit kPa or ug/mL); Digital output: RS-232； Equipped with a two-line text LCD display screen with backlight; Weight 1kg; Size 18cm × 10cm × 18cm

6.Airflow generation and control unit: MFS-2 airflow generation controller, built-in air pump, precision needle valve, mass flow meter; The air pump adopts PID control technology and a brushless motor diaphragm pump; Flow range 75-2000mL/min; The accuracy is 2% of the reading; Resolution 1mL/min; Equipped with a 2-line LCD display screen; Equipped with 0-5V BNC analog signal output; Digital output RS-232; Power supply 12-15VDC, 20-800mA, equipped with AC adapter; Working temperature: 0-50 ℃, no condensation; Weight 3kg; Size 21cm × 15cm × 36cm; Optional MFS-5 airflow controller, flow range 100-4000ml/min

7.Dual channel ultra high precision micro gas flow controller, used in conjunction with high-precision airflow control valve, for precise control of micro airflow when measuring small insects; Multiple models are available, with a minimum control flow range of 0-10mL/min and a maximum control flow range of 0-10L/min; Control port 15 pin D-type interface; Equipped with 0-5V BNC analog signal output; Equipped with a 2-line LCD display screen; Power supply 12-24VDC, with AC adapter; Working temperature: 0-50 ℃, no condensation; Weight 1.4kg; Size 15.2cm × 15.2cm × 7.6cm;

8. Gas path converter: supports automatic or manual switching of 8 gas path channels (including one Baseline channel); Multiple units can be combined into 16 or 24 channels; Response time<50 milliseconds; Supports both push and pull airflow directions; Support stopfloworflow-throughTwo gas path switching modes; Equipped with a 2-line LCD display screen; Power supply 12-15VDC, equipped with AC adapter; Working temperature: 5-45 ℃, no condensation; Weight 3.6kg; Size 20.3cm × 30.5cm × 15.2cm;

9.Secondary sampling unit: equipped with an internal air pump, precision needle valve, and mass flow meter, which can be used for secondary sampling of airflow samples or as a separate gas source; Flow range 5-2000mL/min; The accuracy is 10% of the reading; Resolution 1mL/min; Equipped with a 2-line LCD display screen; Equipped with 0-5V BNC analog signal output; Digital output RS-232; Power supply 12-15VDC, 20-350mA, equipped with AC adapter; Working temperature: 0-50 ℃, no condensation; Weight 1.5kg; Size 16cm × 13cm × 20cm;

10.Data collector: 12 signal input channels, of which 8 analog input channels can measure voltage signals from -5.12V to+5.12V, with 16 bits of digital to analog conversion; Four dedicated temperature input channels are specifically connected to temperature sensors, with a temperature measurement range of -5 to 60 ℃; 2 analog signal output channels, 0-5V, 12 bit resolution; 1 16 bit counter; 8 digital outputs for system control, TTL level; Sampling frequency 100Hz; USB data cable; Equipped with a 2-line LCD display screen; Equipped with 0-5V BNC analog signal output; Digital output RS-232; USB direct power supply; Working temperature: 0-50 ℃, no condensation; Weight 0.9kg; Size 17cm × 15cm × 7cm;

11.Breathing chamber, made of high borosilicate glass, flame polished, Viton ® Ultra low permeability gasket, ultra-low carbon dioxide and water vapor absorption or permeability; There are 3 standard models (other specifications can be customized): RC， Inner diameter 2cm, internal length 3.8cm, volume 30ml, suitable for small insects; RC-2， Inner diameter 4.4cm, internal length 15.2cm, volume 350ml, suitable for large insects and small rodents; RC-3， Inner diameter 7cm, internal length 25.5cm, volume 700ml, suitable for large rodents;

3、 Origin: United States